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Supplemental Material for Humphrey et al., 2020

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posted on 18.02.2020 by Kristen Humphrey, Lisha Zhu, Shirin Hasan, Haniam Maria, Tao Liu, Laura Rusche
We investigated whether sirtuin deacetylases, which repress transcription and require NAD+ for activity, serve as transcriptional rewiring points that facilitate the evolution of potentially adaptive traits. If so, bringing genes under the control of sirtuins could enable organisms to mount appropriate responses to stresses that decrease NAD+ levels. We compared two yeast species, Saccharomyces cerevisiae and Kluyveromyces lactis, and identified sirtuin-regulated genes through a combination of chromatin immunoprecipitation and RNA expression.

History

Article title

Evolution of Distinct Responses to Low NAD+ Stress by Rewiring the Sir2 Deacetylase Network in Yeasts

Manuscript #

GENETICS/2020/303087

Article DOI

10.1534/genetics.119.303087

Licence

Exports