Supplemental Material for Humphrey et al., 2020

We investigated whether sirtuin deacetylases, which repress transcription and require NAD⁺ for activity, serve as transcriptional rewiring points that facilitate the evolution of potentially adaptive traits. If so, bringing genes under the control of sirtuins could enable organisms to mount appropriate responses to stresses that decrease NAD⁺ levels. We compared two yeast species, Saccharomyces cerevisiae and Kluyveromyces lactis, and identified sirtuin-regulated genes through a combination of chromatin immunoprecipitation and RNA expression.