GSA Journals
14 files

Supplemental Material for Higgins et al., 2018

Download all (2.66 MB)
posted on 2018-07-25, 17:20 authored by Douglas A. Higgins, Megan K. Young, Mary Tremaine, Maria Sardi, Jenna M. Fletcher, Margaret Agnew, Lisa Liu, Quinn Dickinson, David PerisDavid Peris, Russell L. Wrobel, Chris Todd Hittinger, Audrey P. Gasch, Steven W. Singer, Blake A. Simmons, Robert Landick, Michael P. Thelen, Trey K. Sato
Supplemental Figures including:
Figure S1. The 378604X strain is highly tolerant to [C2C1im][OAc].
Figure S2. An approach to identify DNA sequences from the tolerant 378604X strain that confer IIL-tolerance.
Figure S3. Overexpression of SGE1 confers tolerance to [C2C1im][OAc] and higher concentrations of [C2C1im]Cl.
Figure S4. The SGE1PLL allele enhances anaerobic conversion of glucose into ethanol in the presence of [C2C1im]Cl.
Figure S5. The 378 strain containing homozygous sge1SLS alleles is sensitive to cationic inhibitors.
Figure S6. The SGE1PLL allele maintains IIL tolerance across a wide pH range.
Figure S7. Myc-tagged SGE1 variant strains also display a range of IL-tolerance phenotypes.
Figure S8. Doxycycline-inducible expression of Sge1 alleles impact cell growth and Sge1 protein levels in the presence of [C2C1im]Cl.
Figure S9. GFP-tagged SGE1 and ILT1 strains display similar IIL-tolerance phenotypes as untagged strains.

Table S1. Deletion of YDR090C sensitizes yeast to [C2C1im]Cl.
Table S2. Engineered S. cerevisiae strains and their genotypes used in this study.

File S1. SGE1 genotypes, phenotypes and metadata of wild S. cerevisiae strains used in this study.

File S2. GATK SNP calls for sequenced wild S. cerevisiae strains.

File S3. SGE1 sequences from additional S. cerevisiae and non-S. cerevisiae strains.


Article title

Natural Variation in Multidrug Efflux Pump SGE1 Underlies Ionic Liquid Tolerance in Yeast

Manuscript #


Article DOI