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Supplemental Material for Gutzmann et al., 2023

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posted on 2023-07-26, 12:52 authored by Daniel J. Gutzmann, Jaxon J. Kramer, Brigid M. Toomey, Cory H. T. Boone, Audrey L. Atkin, Kenneth W. Nickerson

  

File S1 contains the results of the initial screen for differences in farnesol accumulation. The transcription regulator mutants generated by Homann and colleagues (2009) were screened for differences in farnesol as detected in whole cultures, pellets, and supernatants by the GC-FID assay described by Boone and colleagues (2022). For the initial screen, 198 cultures were screened, consisting of 164 mutants from the X plates and 34 replicate controls for the SN152+ parent for a total of 594 GC-FID runs. For each mutant we also report the mean log2 fold change vs the SN152+ control for each of the four analytes as detected in whole cultures, pellets, and supernatants, as well as the supernatant/pellet ratio for each analyte. A legend tab is provided to describe the columns.


File S2 contains the results of the rescreen. For the rescreen, strains were selected because their farnesol values differed from the average values for SN152+ by a log2 fold change of > 1.0. For each of the transcription regulator mutants, both raw data and their means ± SD with n=6, reporting normalized accumulation values for farnesol as detected in whole cultures, pellets, and supernatants. For each mutant we also report the mean log2 fold change vs the SN152+ control for farnesol as detected in whole cultures, pellets, and supernatants, as well as the supernatant/pellet ratio. A legend tab is provided to describe the columns.


File S3 contains the results of the farnesol growth curve. Normalized farnesol accummulation and dry weight measurements were recorded at 12, 18, 24, 36, 48, and 80 hours post inocculation in YPD media. Independent time courses were performed for each TR mutant (n=2) and SN152+ (n=3). A legend tab is provided to describe the columns.

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Article title

Transcriptional regulation of the synthesis and secretion of farnesol in the fungus Candida albicans: Examination of the Homann transcription regulator knockout collection

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