EdskesTOF2Supple.zip (2.48 MB)
Supplemental Material for Edskes, et al., 2021
dataset
posted on 2021-03-04, 18:43 authored by Herman K. Edskes, Emily E. Stroobant, Morgan P. DeWilde, Evgeny E. Bezsonov, Reed B. WicknerThe
Supplementary Information includes extended SILAC methods, strains of S. cerevisiae (Table S1), primers used
(Table S2), proteins increased 1.5-fold or more in abundance in pre9Δ (Table S3), Proteins decreased 1.5-fold or more
in abundance in pre9Δ (Table S4), a
diagram of the proteasome α ring (Fig. S1), evidence that proteasome – related
knockouts do not affect [PSI+] propagation (Fig. S2), evidence that [URE3-1] is
less stable in 779-6A pre9Δ than in
BY241 pre9Δ (Fig. S3), data showing
that levels of Sup35p are not affected by proteasome – related mutations in
strain BY241 (Fig. S4), data showing Btn2p levels in proteasome mutants are
elevated by the presence of [PSI+] (Fig. S5 and S6), measuring the absolute levels of Btn2p and
Cur1p in strain 779-6A by comparison with purified recombinant Btn2-His6 and
Cur1-His6 (Fig. S7), the multiple bands of Cur1p (Fig. S8), the elevation of
Btn2p in tof2 mutants (Fig. S9) and
the absence of a ubiquitin ladder of Btn2p in a pre9Δ strain (Fig. S10).
Table S5 compares protein turnover rates with their levels by SILAC in pre9Δ strains.