1/1
Supplemental Material for Edskes et al., 2018
dataset
posted on 2018-05-16, 16:24 authored by Herman K. Edskes, Maryam Mukhamedova, Bouke K. Edskes, Reed B. WicknerSupplemental Methods_figures_tables.pdf - Provides a detailed description of the culture conditions,
induction of Hermes transposition,
selection of colonies carrying a transposition, extraction of cellular DNA, PCR
amplification and isolation of the junction points between transposon and
chromosomal insertion site, next-generation sequencing of these sites, and
analysis of the data by visual display and by counting insertions per open
reading frame.
Exon Intron Counts.xlsx - Gives the insertions in every yeast open reading frame, distinguishing exons from introns where appropriate.
Sorted Hits.xlsx - Gives prominent hits sorted by functional group, “TY gag-pol Counts.xlsx comparing insertions in the Ty retrotransposons at different locations in the genome.
Count Insertions in ORFs and Introns.txt - The Python program used for counting insertions.
LUGsIGV-InsertDistributions.pptx - Is a slide show of insert distributions in each of 500 genes for which insertions were recovered more frequently in [ure-o] than in [URE3] cultures.
Exon Intron Counts.xlsx - Gives the insertions in every yeast open reading frame, distinguishing exons from introns where appropriate.
Sorted Hits.xlsx - Gives prominent hits sorted by functional group, “TY gag-pol Counts.xlsx comparing insertions in the Ty retrotransposons at different locations in the genome.
Count Insertions in ORFs and Introns.txt - The Python program used for counting insertions.
LUGsIGV-InsertDistributions.pptx - Is a slide show of insert distributions in each of 500 genes for which insertions were recovered more frequently in [ure-o] than in [URE3] cultures.
