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Supplemental Material for Després et al., 2018

posted on 2018-08-09, 18:32 authored by Philippe C Després, Alexandre K Dubé, Lou Nielly-Thibault, Nozomu Yachie, Christian R Landry
Figure S1 shows sequencing of ADE1 loci after Target-AID base editing using double selection. Figure S2 shows Sanger chromatograph titration curve based on different allelic frequencies in the population prior to sequencing. Figure S3 showcases an example of how the lethality of a mutation can influence the observed mutation rate in the population. Figure S4 shows another example of Cas9 mediated chromosomal rearrangement following multiplex editing. Table S1 shows the different plasmids used in this study. Table S2 shows the oligonucleotides used in this study. Table S3 shows a summary of the different gRNA sequences used in this study. Table S4 shows the recipes for synthetic media used in this study.


Article title

Double Selection Enhances the Efficiency of Target-AID and Cas9-Based Genome Editing in Yeast

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