Supplemental Material for Delaney et. al., 2018

Figure S1 shows an analysis of C. elegans H3.3 homologues. Figure S2 contains a summary of deletion and mutation alleles. Figure S3 contains correlation plots of biological replicates for the RNA-seq experiments. Figure S4 shows H3.3 homologue expression during embryogenesis. Figure S5 shows H3.3 homologue expression in the hermaphrodite and male germ line. Figure S6 shows comparison of expression levels of H3.3 homologues. Figure S7 shows that H3.3 proteins are depleted from chromosome X. Figure S8 shows alignment of C. elegans HIRA-1 with homologues from other species. Figure S9 shows morphological defects and reduced brood size of hira-1 mutant worms. Figure S10 shows that HIS-71 and HIS-72 are detectable in the postembryonic somatic tissue even upon hira-1 deletion. Figure S11 shows embryonic lethality and apoptosis in H3.3 null mutant worms. Figure S12 shows the identity and expression of additional H3 homologues in C. elegans. Table S1 lists the C. elegans strains used in this study. Table S2 lists the reagents used for allele generation by CRISPR/Cas9 and quantitative PCR. Table S3 contains the RNA-seq results for embryos. Table S4 contains the RNA-seq results for L1 larvae. Table S5 lists all significantly enriched GO terms.