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Supplemental Material for Colonnetta, et al. 2021

posted on 2021-06-23, 14:26 authored by Megan M. Colonnetta, Juan E. Abrahante, Paul Schedl, Daryl M. Gohl, Girish Deshpande
Figure S1. Examples of defect classes for analysis of patterning gene, mitotic, and cytoskeletal phenotypes.

Figure S2. Zygotic knockdown of CLAMP depletes protein levels in early embryonic nuclei.

Figure S3. Zygotic knockdown of Bicoid depletes protein levels in the anterior of bcdi embryos.

Figure S4. RNA-seq reveals more decreased targets in early vs. late datasets.

Figure S5. Localization of CLAMP and Zld in genomic regions containing the pair-rule genes eve and run and the gap genes btd and gt.

Figure S6. Levels of CLAMP protein levels and patterning gene proteins correlate in clampi embryos.

Figure S7. Transcription of the pair-rule gene runt is disrupted in clampi1 or zldi embryos.

Figure S8. Zygotic clamp knockdown results in variable decrease in Buttonhead protein levels.

Figure S9. CLAMP and Zelda are required for proper expression of the gap gene gt.

Figure S10. Levels of Sxl protein are decreased in female compromised for CLAMP activity.

Figure S11. CLAMP occupancy shifts during the minor wave of ZGA.

Table S1. Decreased targets in both CLAMP knockdown collections at 0-1.5 hr and 1.5-3 hr.

Table S2. Comparison of decreased zygotic genes between previously published datasets and our RNA-seq data from zygotic knockdown embryos.

Table S3. Maternal/zygotic classification of target genes decreased in 0-1.5 hr RNA-seq datasets.

Table S4. CLAMP binding genes near the TSS increases at NC11 and is maintained throughout later nuclear cycles.

Table S5. Decreased transcripts (by at least 2-fold) in 0-1.5 hr collections of clampi1, zldi, and zld/clampi1 embryos.

Reagent Table details all reagents used in the manuscript.

Colonnetta et al. CLAMP_Genetics_Supplement contains full figure legends.


Article title

CLAMP regulates Zygotic Genome Activation in Drosophila embryos