Supplemental Material for Chen et al., 2020

dataset

posted on 2020-06-09, 15:12 authored by Lijiao Chen, Xingyun Shi, Bo Nian, Shuangmei Duan, Bin Jiang, Xinghua Wang, Caiyou Lv, Guanghui Zhang, Yan Ma, Ming Zhao

HPLC chromatography for determation of anthocyanins and catechins (Figure S1 and Table S1, Supplemental data 1).The number of ZJ (Purple digital) and YK (Green digital) isoforms that are enriched in terms of molecular function, cellular component, and biological process based on GeneOntology (GO) analysis(Figure S2).Gene annotation of KEGG pathways involved in biosynthesis of ZJ and YK. The isoforms of ZJ and YK have been gathering in KEGG database，and the numbers have been shown and compared(Figure S3).The relative expression quantity of genes and their AS transtrips in ZJ and YK one leaf and two buds to ZJ Mature leaves based on qRT-PCR studies (Figure S4) and the Primers used in the study have been listed (Table S2).The number of isoforms in anthocyanidin biosynthesis of YK and ZJ (Supplemental data 2 and 3).The GeneOntology (GO) annotation of ZJ and YK PacBio Iso-seq isoforms (Supplemental data 4) and The KEGG annotation of ZJ and YK PacBio Iso-seq isoforms(Supplemental data 5).The classification of AS evens happened in ZJ and YK (Supplemental data 6).The number of AS evens happened in anthocyanidin biosynthesis in YK and ZJ (Supplemental data 7).The relative expression quantity of genes and their AS transtrips in ZJ and YK one leaf and two buds to ZJ Mature leaves (Supplemental data 8).The coefficient of association matrix between chemical composition contents and gene expressions(Supplemental data 9)

History

Article title

Alternative Splicing Regulation of Anthocyanin Biosynthesis in Camellia sinensis var. assamica Unveiled by PacBio Iso-Seq

Manuscript #

G3/2020/401451

Article DOI

10.1534/g3.120.401451

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Keywords

anthocyanins PacBio Iso-Seq alternative splicing events Plant Biology

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