Supplemental Material for Boone et al., 2020

Table S1 Primers for strain construction and confirmation. Primers for DJ-PCR-based fluorescent tagging were designed as previously described (Hammond et al. 2011b). The center DJ-PCR fragment for nup120-mCherry tagging was obtained from plasmid pEB1.1, which is similar to pTH1112.8 except that mCherry (Castro-Longoria et al. 2010) is present instead of yfpn. The center DJ-PCR fragment for yfpn-cbp20 and yfpn-sad-8 tagging was obtained from plasmid pNTYN-2, which is similar to pTH1123.1 except that nat1 (Lichius and Read 2010) is present instead of hph.