Supplemental Material for Boone et al., 2020
datasetposted on 2020-04-24, 16:28 authored by Erin C. Boone, Hua Xiao, Michael M. Vierling, Logan M. Decker, Victor T. Sy, Rana F. Kennedy, Marilyn A. Bonham, Shannon F. Schmitz, Annie M. John, Thomas M. Hammond, Patrick K.T. Shiu
Table S1 Primers for strain construction and confirmation. Primers for DJ-PCR-based fluorescent tagging were designed as previously described (Hammond et al. 2011b). The center DJ-PCR fragment for nup120-mCherry tagging was obtained from plasmid pEB1.1, which is similar to pTH1112.8 except that mCherry (Castro-Longoria et al. 2010) is present instead of yfpn. The center DJ-PCR fragment for yfpn-cbp20 and yfpn-sad-8 tagging was obtained from plasmid pNTYN-2, which is similar to pTH1123.1 except that nat1 (Lichius and Read 2010) is present instead of hph.