Supplemental Material for Benbow, Jermiin, and Doohan, 2019

Supplementary file 2 is a fasta file multiple sequence alignment (created with Clustal Omega) when uninformative sites masked (removed). Quality scores for the original alignment were generated with AliStat, and sites in the alignment that had a completemess score of < 0.4 were removed.

Supplementary file 3 is a fasta file multiple sequence alignment (created with Clustal Omega) with imcomplete sites removed (as per supplementary file 2), and sequences removed to avoid the alignment containing any pairs of sequences that contain no overlap. This is final alignment that was used for creation of the wheat serpin phylogenetic tree.

Supplement figures and tables file contains:

Tables S1 and S2 - tables that give details of the publicly available RNAseq data that was used to profile the expression of the wheat serpin genes.

Figure S1 - A heatmap showing differential expression of wheat serpins in the grain at three time points post inoculation with Fusarium graminearum in two wheat cultivars.

Figure S2 - A phylogenetic tree of a sub-clade of wheat serpins that contains five Fusarium graminearum reponsive serpin genes.