Arlt_et_al_Supplemental_Revision.pdf (1.26 MB)

Supplemental Material for Arlt et al., 2020

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posted on 06.04.2020 by Martin F. Arlt, Michele A. Brogley, Evan Stark-Dykema, Yueh-Chiang Hu, Jacob L. Mueller

Supplemental Table 1 contains RT-PCR primer sequences. Supplemental Table 2 contains primer pairs used in RT-PCR assays. Supplemental Table 3 contains oligos used to generate and genotype transgenic mice. Supplemental Figure 1 displays a self-symmetry triangular dot plot of the Srsx-containing ampliconic region on the X. Supplemental Figure 2 is a dot plot of DNA sequence identity between two Srsx-containing X amplicons. Supplemental Figure 3 shows low level of expression from the Y of sequences with homology to Laidx. Supplemental Figure 4 shows Laidx expression exclusively in the testis. Supplemental Figure 5 shows all RT=PCR assays used to characterize Ladix. Supplemental Figure 6 displays IUPred2A output with high probability of disorder across the LAIDX protein. Supplemental Figure 7 shows dotplots comparing mouse and rat Laidx genes. Supplemental Figure 8 is a dotplot comparing rat Laidx to the rat Y chromosome. Supplemental Figure 9 contains dotplots showing similarity of rat Laidx to a Clonorchis gene. Supplemental Figure 10 displays Periodic-acid Schiff stained testis histology from WT and Laidx-/Y males demonstrating normal development and morphology.


Article title

Genomic Structure, Evolutionary Origins, and Reproductive Function of a Large Amplified Intrinsically Disordered Protein-Coding Gene on the X Chromosome (Laidx) in Mice

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