Supplementary Material for Wernick et al. 2019

<div>Supplementary figures show the following; S1 and S2: relative and competitive fitness of <i>gas-1</i> RC lines, respectively; S3: normalized mtDNA copy number for all <i>C. elegans</i> lines; S4: expected and observed percentages of <i>gas-1</i> RC line genic mtDNA mutations; S5: chromatograms depicting evolutionary trajectories of two mtDNA variants; S6: GO enrichments and interactome results for <i>gas-1</i> RC line mutations; S7: GO term categorizations for <i>gas-1</i> RC line SNPs. </div><div><br></div><div>Supplementary tables contain the following data; S1: Illumina HiSeq run statistics; S2: ANOVA results for <i>C. elegans</i> life-history traits; S3: results of mtDNA copy number normalization; S4: <i>gas-1</i> G0 mutations reverted to wildtype alleles within RC lines; S5: mtDNA SNPs; S6: nDNA SNPs; S7: <i>gas-1</i> RC line SNPs occurring within the <i>gas-1</i> interactome. </div><div><br></div><div>File S1 shows the frequencies within each <i>gas-1 </i>RC line for all mtDNA positions in which a variant was identified.</div><div><br></div><div>File S2 shows the frequencies within each <i>gas-1</i> MA line for the <i>fox-1</i> single-basepair reversion discovered within three <i>gas-1</i> RC lines.</div><div><br></div><div>File S3 contains custom code for simulations reported in Figure S5. </div><div><br></div><div>File S4 contains a complete accounting of <i>gas-1</i> RC G0 enrichment results. <br></div>