Supplementary Material for Wernick et al. 2019

Supplementary figures show the following; S1 and S2: relative and competitive fitness of gas-1 RC lines, respectively; S3: normalized mtDNA copy number for all C. elegans lines; S4: expected and observed percentages of gas-1 RC line genic mtDNA mutations; S5: chromatograms depicting evolutionary trajectories of two mtDNA variants; S6: GO enrichments and interactome results for gas-1 RC line mutations; S7: GO term categorizations for gas-1 RC line SNPs.

Supplementary tables contain the following data; S1: Illumina HiSeq run statistics; S2: ANOVA results for C. elegans life-history traits; S3: results of mtDNA copy number normalization; S4: gas-1 G0 mutations reverted to wildtype alleles within RC lines; S5: mtDNA SNPs; S6: nDNA SNPs; S7: gas-1 RC line SNPs occurring within the gas-1 interactome.

File S1 shows the frequencies within each gas-1 RC line for all mtDNA positions in which a variant was identified.

File S2 shows the frequencies within each gas-1 MA line for the fox-1 single-basepair reversion discovered within three gas-1 RC lines.

File S3 contains custom code for simulations reported in Figure S5.

File S4 contains a complete accounting of gas-1 RC G0 enrichment results.