Supplemental Material for Zhang et al., 2018
Figure S1 RNAi knockdown of Class 3 and 4 genes led to defects in oocyte development. Ovaries dissected from 3-5 days old control or knockdown females were stained for DNA (red) using propidium iodide. A summary of the ovarian phenotypes can be found in Table S3. Images are arranged according to observed stage of oogenesis defect, from early stage to late stage. Scale bar: 50 µm.
Figure S2. Maternal knockdown of 12 genes led to reduction and elimination of egg hatchability that recapitulated previously reported maternal effect phenotypes. 2-4 hr old embryos from knockdown females, stained for tubulin (green) and DNA (red). For each set of images, the image on the right shows a close-up view of the spindle (marked by the arrow on the left). Images are organized in alphabetical order. The AttP2 panel, the control for comparison, is the same as in Fig. 2. Scale bar: oocyte image 50 µm, enlarged image 10 µm.
Figure S3. Fertilization was not significantly impacted in the early arrest knockdown embryos of the 27 genes Sperm tail (green; arrow) was detected in the stage 1-2 arrested embryos produced by RNAi females for 27 genes. The images are ordered in alphabetical order, by gene name. Scale bar: 50 µm.
S4. Translation of smg in unfertilized eggs produced by control and RNAi
females of 27 early maternal effect candidates. Among the 27 genes examined, Smg protein
was detected in the activated eggs produced by RNAi females of 26 genes.
Translation of Smg protein was not detected in the activated eggs produced by plu knockdown females.
Table S1 - S7