Supplemental Material for Tamm, Kisly, and Remme, 2019

Table S1 lists the S. cerevisiae strains used in this study. Material and Methods chapter describes the Visualization of ribosome and r-protein structures, Preparation of 80S ribosomes for mass spectrometric analysis and LC-MS/MS analysis. Figure S1 shows the structures of the r-proteins eL19 and eL24. Figure S2 illustrates the strategy for strain construction. Figure S3 shows the analysis of global levels of translation of eL24 mutants. Figure S4 shows the mass spectrometric analysis of ribosomes from Archaea-like mutants. Figure S5 shows the analyzed peptides originated from the r-protein eL19. Figure S6 shows the analyzed peptides originated from the r-protein eL24.