Supplemental Material for Santiago-Cartagena et al., 2019

<p><b>Table S1.</b> Strains used in this study.</p> <p> </p> <p><b>Table S2.</b> Oligonucleotides used in this study.</p><p><strong>Table S3.</strong> Percentages of positive interactors for Wsc1p and Mid2p identified by two independent iMYTH screens performed at 37<sup>o</sup>. </p> <p> </p> <p><b>Figure S1.</b> NubG/I test for integrated C-tagged Wsc1p and Mid2p.</p> <p> </p> <p><b>Figure S2.</b> Localization of Wsc1p and Mid2p.</p> <p> </p> <p><b>Figure S3.</b> Validation of MYTH-tagged Mid2p and Wsc1p expression by Western blot.</p> <p> </p> <p><b>Figure S4.</b> WSC1 and MID2 - iMYTH interactome showing only interactors identified in the iMYTH screen at 30<sup>o</sup>. </p> <p> </p> <p><b>Figure S5.</b> Growth curve analysis of single and double mutants exposed at different concentrations of Hydrogen Peroxide.</p> <p> </p> <p><b>Figure S6.</b> Growth curve analysis of single and double mutants exposed at different concentrations of Caspofungin.</p> <p> </p> <p><b>Figure S7.</b> Western blot analysis showing the phosphorylation of Slt2p in single and double mutant strains treated with 1mM hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) for 1 hr at 27°.</p> <p> </p> <p><b>Figure S8.</b> Western blot analysis showing the phosphorylation of Slt2p in single and double mutant strains treated with 75ng/ml Caspofungin for 1 hr at 27°.</p> <p> </p> <p><b>Figure S9.</b> Network graph of the Wsc1p and Mid2p interactome identified by iMYTH screen at 37<sup>o</sup>.</p> <p> </p> <p><b>Figure S10. </b>A representative drop dilution assay<b> </b>of sensor and interactor null mutants exposed to stress conditions.<b></b></p>