Supplemental Material for Santiago-Cartagena et al., 2019

Table S1. Strains used in this study.

Table S2. Oligonucleotides used in this study.

Table S3. Percentages of positive interactors for Wsc1p and Mid2p identified by two independent iMYTH screens performed at 37o.

Figure S1. NubG/I test for integrated C-tagged Wsc1p and Mid2p.

Figure S2. Localization of Wsc1p and Mid2p.

Figure S3. Validation of MYTH-tagged Mid2p and Wsc1p expression by Western blot.

Figure S4. WSC1 and MID2 - iMYTH interactome showing only interactors identified in the iMYTH screen at 30o.

Figure S5. Growth curve analysis of single and double mutants exposed at different concentrations of Hydrogen Peroxide.

Figure S6. Growth curve analysis of single and double mutants exposed at different concentrations of Caspofungin.

Figure S7. Western blot analysis showing the phosphorylation of Slt2p in single and double mutant strains treated with 1mM hydrogen peroxide (H2O2) for 1 hr at 27°.

Figure S8. Western blot analysis showing the phosphorylation of Slt2p in single and double mutant strains treated with 75ng/ml Caspofungin for 1 hr at 27°.

Figure S9. Network graph of the Wsc1p and Mid2p interactome identified by iMYTH screen at 37o.

Figure S10. A representative drop dilution assay of sensor and interactor null mutants exposed to stress conditions.