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Supplemental Material for Saltzman et al., 2018

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posted on 2018-09-04, 19:37 authored by Arneet L Saltzman, Mark Soo, Reta Aram, Jeannie T. Lee

Figure S1. Chromo domain-containing proteins in C. elegans.

Figure S2. Additional comparison of histone peptide binding specificity of CEC1, CEC-6 and CEC-3 chromo domain containing proteins.

Figure S3. Developmental and spatial expression patterns of cec-1, cec-6 and cec-3.

Figure S4. Additional immunofluorescence of 3xHA-tagged CEC-1 and CEC-6 in dissected germlines.

Figure S5. Additional characterization of deletion strains.

Figure S6. Additional images of germline defects in sterile late-generation cec-3Δ;cec-6Δ animals.

Figure S7. cec-3Δ;cec-6Δ animals do not show increased RAD-51 foci in the mitotic region of the germline.

Table S1. Description of strains and alleles used in this study.

Table S2. Quantification of relative binding of CEC-1 and CEC-6 to histone peptide arrays.

History

Article title

Multiple Histone Methyl-Lysine Readers Ensure Robust Development and Germline Immortality in Caenorhabditis elegans

Manuscript #

GENETICS/2018/301518

Article DOI

10.1534/genetics.118.301518

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