Supplemental Material for Robinson et al., 2019

<p><b>Supplementary Table 1. Average fold-change for GO categories that were significantly enriched with genes down- and up-regulated in expression in response to acute stress for control (CO), embryonic (E), post-hatch (PH) and embryonic post-hatch (EPH) treated fish. </b>Significance was tested using 1-tailed chi-square test with Yates’ correction for continuity, <i>P</i><0.05.<b> </b></p> <p><b> <b>Supplementary Figure 1. Volcano plots of adjusted <i>P</i>-values (<i>P</i><sub>adj</sub>) and log fold change in expression (log<sub>2</sub>FC) in response to acute stress (ie. 0 versus 3 hours post stress). </b>Separate plots are for control (A) embryonic (B) post hatch (C) and embryonic post hatch (D) experimental treatments. Red points show genes with <i>P</i><sub>adj </sub>< 0.05 while green points show genes with <i>P</i><sub>adj </sub>< 0.05 and log<sub>2</sub>FC > 1.</b></p> <p><b>Supplementary Figure 2. Pearson correlation analyses of fold difference in expression between treatments and response to acute stress after treatments. </b>CO, E, PH and EPH refer to control, embryo, post-hatch and embryo post-hatch treatments respectively. </p> <p><b>Supplementary Figure 3. Box plots of changes in the expression of T-cell receptor (transcript XLOC_015821) for control (CO), embryonic (E), post-hatch (PH) and embryonic post-hatch (EPH) treated fish and map of methylation differences between treatments 0 hrs pre-acute stress (stars). </b>The gene expression response for EPH treated fish (from 0 to 3 hrs after acute stress) is shown as green bars positioned at the start and end of the gene transcript.<b> </b></p> <p><b>Supplementary Figure 4. Box plots of changes in the expression of Toll-like receptor 3 (transcript XLOC_022410) for control (CO), embryonic (E), post-hatch (PH) and embryonic post-hatch (EPH) treated fish and map of methylation differences between treatments 0 hrs pre-acute stress (stars). </b>The gene expression response for EPH treated fish (from 0 to 3 hrs after acute stress) is shown as green bars positioned at the start and end of the gene transcript.<b> </b></p> <p><b>Supplementary Figure 5. Box plots of changes in the expression of Immunoglobulin H locus B (transcript XLOC_022187) for control (CO), embryonic (E), post-hatch (PH) and embryonic post-hatch (EPH) treated fish and map of methylation differences between treatments 0 hrs pre-acute stress (stars). </b>The gene expression response for EPH treated fish (from 0 to 3 hrs after acute stress) is shown as green bars positioned at the start and end of the gene transcript.<b> </b></p> <p><b>Supplementary Figure 6. Box plots of changes in the expression of ATP Binding Cassette (ABC membrane transporters) (transcript XLOC_014577) for control (CO), embryonic (E), post-hatch (PH) and embryonic post-hatch (EPH) treated fish and map of methylation differences between treatments 0 hrs pre-acute stress (stars). </b>The gene expression response for EPH treated fish (from 0 to 3 hrs after acute stress) is shown as green bars positioned at the start and end of the gene transcript.<b> </b></p><p><br></p><p>File (.gff) giving annotation used to derive the gene counts. The creation of this file is described in Moghadam et al. (2017).<br></p>