Supplemental Material for Meissner et al., 2018

File S1. FISH probes for Gad1 (CF594; red), vGlut (Cy5; blue), and ChAT (DL550; green) mRNAs in the brain. Five 63x confocal stacks of different regions of the brain were imaged with the same parameters, corrected for lens-distortion and chromatic aberration (Bogovic et al. 2018) and stitched together (Yu and Peng 2011). Movies were rescaled in x & y and compressed. Pixel size at imaging, 0.19 x 019 x 0.38 µm. Pixel size after scaling, 0.38 x 0.38 x 0.38 µm. Scale bar, 50 µm.

File S2. As in File S1 but showing the ventral nerve cord.

File S3. As in File S1, with FISH probes for SerT (CF594; red), pale (Cy5; blue), and Tβh (DL550; green) mRNAs in the brain.

File S4. As in File S3 but showing the ventral nerve cord.

File S5. As in File S1, with FISH probes for Hdc (DL550; green) and Tdc2 (Cy5; magenta) mRNAs in the brain.

File S6. As in File S5 but showing the ventral nerve cord.

File S7. FISH probes for Gad1 (CF594; red), vGlut (Cy5; blue), and ChAT (DL550; green) mRNAs in a SS02565, UAS-HaloTag (white) brain. Boxed area indicates the brain region shown in Figure 3E.

File S8. FISH probe for pale (Cy5; green) mRNA in a TH-GAL4, UAS-HaloTag (white) brain, corresponding to Figure 2B.

File S9. FISH probe for pale (Cy5; green) mRNA in a R58E02-GAL4, UAS-HaloTag (white) brain, corresponding to Figure 2C.

File S10.FISH probe for SerT (Cy5; magenta) mRNA with a mouse anti-serotonin antibody (AF568 anti-mouse; green) in a wild-type brain, corresponding to Figure S2C. Scale bar, 20 µm.

File S11. FISH probes for Tdc2 (Cy5; magenta) and Tβh (Cy3; green) mRNAs in a wild-type brain, corresponding to Figure 2F. Scale bar, 20 µm.

Protocol. FISH Protocol

Table S1. FISH Probe Sequences

Figure 3.zip. Original LSM

Figure S5.zip. Original LSM

Figure S6.zip. Original LSM

Bogovic, J. A., H. Otsuna, L. Heinrich, M. Ito, J. Jeter et al., 2018 An unbiased template of the Drosophila brain and ventral nerve cord. . BioRxiv DOI: https://doi.org/10.1101/376384.

Nassel, D. R., 1999 Histamine in the brain of insects: A review. Microscopy Research and Technique 44: 121-136.

Yu, Y., and H. C. Peng, 2011 Automated High Speed Stitching of Large 3d Microscopic Images. 2011 8th Ieee International Symposium on Biomedical Imaging: From Nano to Macro: 238-241.