Supplemental Material for Meissner et al., 2018

<p></p><p></p><p>File S1. FISH probes for <i>Gad1</i> (CF594; red), <i>vGlut</i> (Cy5; blue), and <i>ChAT</i> (DL550; green) mRNAs in the brain. Five 63x confocal stacks of different regions of the brain were imaged with the same parameters, corrected for lens-distortion and chromatic aberration (Bogovic <i>et al.</i> 2018) and stitched together (Yu and Peng 2011). Movies were rescaled in x & y and compressed. Pixel size at imaging, 0.19 x 019 x 0.38 µm. Pixel size after scaling, 0.38 x 0.38 x 0.38 µm. Scale bar, 50 µm.</p><p></p> <p></p><p>File S2. As in File S1 but showing the ventral nerve cord.</p><p></p> <p></p><p>File S3. As in File S1, with FISH probes for <i>SerT</i> (CF594; red), <i>pale</i> (Cy5; blue), and <i>Tβh</i> (DL550; green) mRNAs in the brain.</p><p></p> <p></p><p>File S4. As in File S3 but showing the ventral nerve cord.</p><p></p> <p></p><p>File S5. As in File S1, with FISH probes for <i>Hdc</i> (DL550; green) and <i>Tdc2</i> (Cy5; magenta) mRNAs in the brain.</p><p></p> <p></p><p>File S6. As in File S5 but showing the ventral nerve cord.</p><p></p> <p></p><p>File S7. FISH probes for <i>Gad1</i> (CF594; red), <i>vGlut</i> (Cy5; blue), and <i>ChAT</i> (DL550; green) mRNAs in a <i>SS02565</i>, <i>UAS-HaloTag</i> (white) brain. Boxed area indicates the brain region shown in Figure 3E.</p><p></p> <p></p><p>File S8. FISH probe for <i>pale</i> (Cy5; green) mRNA in a <i>TH-GAL4</i>, <i>UAS-HaloTag</i> (white) brain, corresponding to Figure 2B.</p><p></p> <p></p><p>File S9. FISH probe for <i>pale</i> (Cy5; green) mRNA in a <i>R58E02-GAL4</i>, <i>UAS-HaloTag</i> (white) brain, corresponding to Figure 2C.</p><p></p> <p></p><p>File S10.FISH probe for <i>SerT</i> (Cy5; magenta) mRNA with a mouse anti-serotonin antibody (AF568 anti-mouse; green) in a wild-type brain, corresponding to Figure S2C. Scale bar, 20 µm.</p><p></p> <p></p><p>File S11. FISH probes for <i>Tdc2</i> (Cy5; magenta) and <i>Tβh</i> (Cy3; green) mRNAs in a wild-type brain, corresponding to Figure 2F. Scale bar, 20 µm.</p><p></p> <p></p><p>Protocol. FISH Protocol</p><p></p> <p></p><p>Table S1. FISH Probe Sequences</p><p></p> <p></p><p>Figure 3.zip. Original LSM</p><p></p> <p></p><p>Figure S5.zip. Original LSM</p><p></p> <p></p><p>Figure S6.zip. Original LSM</p><p></p> <p></p><p>Bogovic, J. A., H. Otsuna, L. Heinrich, M. Ito, J. Jeter et al., 2018 An unbiased template of the Drosophila brain and ventral nerve cord. . BioRxiv DOI: https://doi.org/10.1101/376384.</p><p></p> <p></p><p>Nassel, D. R., 1999 Histamine in the brain of insects: A review. Microscopy Research and Technique 44: 121-136.</p><p></p> <p></p><p>Yu, Y., and H. C. Peng, 2011 Automated High Speed Stitching of Large 3d Microscopic Images. 2011 8th Ieee International Symposium on Biomedical Imaging: From Nano to Macro: 238-241.</p><p></p><br><p></p>