Supplemental Material for Magwanga et al., 2018

Supplementary Fig. S1: Phylogenetic tree, gene structure and motif compositions of LEA2 genes in upland cotton. The phylogenetic tree was constructed using MEGA 7.0. Exon/intron structures of LEA genes in upland cotton, exons introns and up/down-stream were represented by yellow boxes, black lines and blue boxes, respectively. Protein motif analysis represented by different colours, and each motif represented by number

Supplementary Fig. S2: conserved motifs in upland cotton, G. hirsutum LEA2 proteins. The conserved motifs were obtained using MEME program. The color scheme of the logo indicates amino acid types. Polar: green=uncharged; blue=+vely charged; red=-vely charged; Non-polar: violet/purple=aliphatic. As described by Dure, 2001; Supplementary Fig. S3: Alignment of the LEA2 protein sequences of Upland cotton, Gossypium hirsutum. Amino acid sequences were aligned using the ClustalW2 algorithm. Dashes indicate gaps introduced for optimal alignment. The typical LEA2 sequence elements are bound in boxes in different colours: K segment–red box; Y segment–green; S segment–black; E segment–gray; R segment–purple; D segment–yellow

Supplementary Fig. S4. LEA2 genes distribution in A and D cotton chromosomes: Chromosomal position of each LEA2 genes was mapped according to the upland cotton genome. The chromosome number is indicated at the top of each chromosome

Supplementary Fig. S5: LEA2 genes distribution in upland cotton chromosomes. Chromosomal position of each LEA2 genes was mapped according to the upland cotton genome. The chromosome number is indicated at the top of each chromosome

Supplementary Table S1: List of primers used for upland cotton, G. hirsutum LEA2 genes expression analysis under drought stress

Supplementary Table S2: Conserved domain analysis of LEA2s proteins identified from upland cotton using CDD tool from NCBI

Supplementary Table S3: Transmembrane domains in LEA2s identified from upland cotton, using TMHMM and SOSUI servers. ExpAA: The expected number of amino acids in transmembrane helices. First60: The expected number of amino acids in transmembrane helices in the first 60 amino acids of the protein. MP: Membrane protein; TMH: The number of predicted transmembrane helices; Supplementary Table S4: Subcellular location as determined by TargetP and Pprowler prediction tools. SP-secretory pathways; C-chloroplast;-any other location; Supplementary Table S5: Cis element analysis of putative LEA 2 promoters related to drought stress

Supplementary Table S6: LEA2 genes and miRNA targets