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Supplemental Material for Lange, Suleman, and Srayko, M., 2019

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posted on 2019-04-24, 14:08 authored by Karen Lange, Aly Suleman, Martin Srayko
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Figure S1 shows anti-Ska immunofluorescence in one-cell embryos treated with control or ska-1 RNAi. Figure S2 shows KLP-7-GFP in the one-cell embryo is not altered by ska-1(RNAi) or ska-3(RNAi). Figure S3 shows Ska mutations do not restore microtubule levels in rsa-1(or598) embryos. Figure S4 shows TPXL-1-GFP at the centrosome is not altered by ska-1(RNAi). Figure S5 shows Ska is required to form a tight metaphase plate. Figure S6 shows the rsa-1(or598) spindle-collapse phenotype is partially rescued by loss of Ska function. Figure S7 shows the rsa-1(or598) spindle-collapse phenotype is partially rescued by loss of KMN kinetochore components and loss of EBP-2. Table S1 contains information on C. elegans strains used in this study. Table S2 contains information on cloning primers used in this study.

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Article title

Kinetochore Recruitment of the Spindle and Kinetochore-Associated (Ska) Complex is Regulated by Centrosomal PP2A in Caenorhabditis elegans

Manuscript #

GENETICS/2019/302105

Article DOI

10.1534/genetics.119.302105

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