Supplemental Material for Kaplan et al., 2018

Complete normalized expression data set. Raw data is available upon request.



Supp Data File 1. Complete statistics for regulation of all genes during starvation and recovery by daf-16 and temperature.


Supp Data File 2. Complete normalized expression data set. Raw data is available upon request.


Figure S1 - complementing Fig. 2. Insulin-like signaling regulates expression of genes comprising the insulin-like signaling pathway during L1 starvation. Transcript abundance (arbitrary units) is plotted during L1 starvation at three different temperatures in five different genotypes with various levels of insulin-like signaling activity. In addition to daf-2/InsR and components of the PI3K pathway, the transcriptional effectors of signaling, daf-16/FoxO and skn-1/Nrf, are plotted as well as the known DAF-16 target sod-3. Note that daf-16 expression was not detected in daf-16 mutants. Each gene plotted was significantly affected by daf-16 (see Tables 1, S1 and Supp. Data). Error bars reflect the SEM of two or three biological replicates.

Figure S2 - complementing Fig. 3. Insulin-like signaling and temperature regulate expression of the majority of insulin-like genes during L1 starvation. Transcript abundance (arbitrary units) is plotted during L1 starvation at three different temperatures in five different genotypes with various levels of insulin-like signaling activity. Of 28 reliably detected insulin-like genes, 25 were significantly affected by daf-16 and are plotted (same genes as in Fig. 3). 21 were significantly affected by temperature (all but ins-2, 9, 12, 16, 21, 29, 34). ins-10 was affected by temperature but not daf-16 and is not plotted here (see Tables 1, S1 and Supp. Data). Error bars reflect the SEM of two or three biological replicates.

Table S1. Summary of genes whose expression is affected by temperature during L1 starvation.