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Supplemental Material for Jacobsen et al., 2019

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posted on 2019-07-01, 14:55 authored by Elsie C. Jacobson, Lekha Jain, Mark H. Vickers, Ada L. Olins, Donald E. Olins, Jo K. Perry, Justin M. O’Sullivan

Additional file 1. Supplementary tables 1-22. 1. Significantly differentially expressed genes in promyelocytes treated with TNF-a, differentiated into granulocytes and macrophages, and granulocytes treated with TNF-a. 2. RT-qPCR and RNA-seq of CDK1, TNF, and VCAM1. 3-21. Functional analysis summary tables of GSEA, GO, KEGG, and hallmark terms enriched in subsets of differentially expressed genes. 22. Primers and probes used for RT-qPCR validation.


Supplementary figure 1. PCA of VST-normalized transcript counts show conditions cluster together in A) promyelocytes treated with TNF-a, B) granulocytes treated with TNF-a, and C) promyelocytes differentiated into granulocytes and macrophages.

History

Article title

TNF-alpha Differentially Regulates Cell Cycle Genes in Promyelocytic and Granulocytic HL-60/S4 Cells

Manuscript #

G3/2019/400361

Article DOI

10.1534/g3.119.400361