Supplemental Material for Hoyos-Manchado et al., 2020

Figure S1. Representative spot test of isolated mutants. (A) Example strains of ENU-induced inactivating mutations in kanMX6 (3) and hphMX6 (2) markers were grown until mid-log phase. A single spot of each mutant is plated in regular rich medium (YES) and YES supplemented with G418 or hygromycin B to show respective resistance loss. Strains are labelled by the amino acid change indicated in table 3. (B) Five-fold serial dilutions of wild type and four temperature sensitive mutant strains isolated in this screen were spotted on YES plates at 25º and 36º. (C) Five-fold serial dilutions of parental prototroph strain and four auxotrophic strains deriven by ENU mutagenesis were spotted in YES (left), minimal medium without any supplement (second left), minimal medium lacking just adenine (second right), and minimal medium lacking just histidine (right). The two indicated mutations in ade10 gene make those strains dependent on adenine and histidine with a different expressivity.