Supplemental Material for Higgins et al., 2018

<div>Supplemental Figures including:</div>Figure S1. The 378604X strain is highly tolerant to [C2C1im][OAc].<br>Figure S2. An approach to identify DNA sequences from the tolerant 378604X strain that confer IIL-tolerance.<br>Figure S3. Overexpression of SGE1 confers tolerance to [C2C1im][OAc] and higher concentrations of [C2C1im]Cl.<br>Figure S4. The SGE1PLL allele enhances anaerobic conversion of glucose into ethanol in the presence of [C2C1im]Cl.<br>Figure S5. The 378 strain containing homozygous sge1SLS alleles is sensitive to cationic inhibitors.<br>Figure S6. The SGE1PLL allele maintains IIL tolerance across a wide pH range.<br>Figure S7. Myc-tagged SGE1 variant strains also display a range of IL-tolerance phenotypes.<br>Figure S8. Doxycycline-inducible expression of Sge1 alleles impact cell growth and Sge1 protein levels in the presence of [C2C1im]Cl.<br>Figure S9. GFP-tagged SGE1 and ILT1 strains display similar IIL-tolerance phenotypes as untagged strains.<div><br></div><div>Table S1. Deletion of YDR090C sensitizes yeast to [C2C1im]Cl.<br>Table S2. Engineered S. cerevisiae strains and their genotypes used in this study.<br></div><div><br></div><div><div>File S1. <i>SGE1</i> genotypes, phenotypes and metadata of wild <i>S. cerevisiae</i> strains used in this study.</div><div><br></div><div>File S2. GATK SNP calls for sequenced wild <i>S. cerevisiae</i> strains.</div><div><br></div><div>File S3. <i>SGE1</i> sequences from additional <i>S. cerevisiae</i> and non-<i>S. cerevisiae</i> strains.</div></div>