Supplemental Material for González-Morales et al., 2019

Figure S1. Radial phylogenetic tree of insect species from the 1Kite project with Zasp52, Zasp66, and Zasp67 orthologs highlighted. The tip branches correspond to all the genomes analyzed (see Table S1), the colored circles denote the presence of Zasp52, Zasp66, and Zasp67 orthologs, respectively. Related to Figure 1A.

Figure S2. Localization of Zasp52 in Zasp67 mutant. Confocal microscopy images of control and Zasp67 mutant indirect flight muscles expressing an endogenous version of Zasp52 tagged with mCherry. The images have been colored according to the pixel intensity values. The intensity scale is shown on the right. Related to Figure 6.

Figure S3. Quantification of Zasp67 phenotype in indirect flight muscles. (A-C) High magnification confocal images from single sarcomeres showing a normal-looking Z-disc in A, an extended Z-disc in B, and a broken Z-disc in C. (D-F) Dot plots of the ratio of different Z-disc phenotypes in control and Zasp67 deficient flies. Each dot represents the number of Z-discs with a clear phenotype divided by the total number of Z-discs present in a sample indirect flight muscle image. Below the plots are the p-values adjusted by Bonferroni correction for selected genotype pairs. The abnormal Z-disc counts are shown in D, the extended Z-disc counts in E, and the broken Z-disc counts in F. Related to Figure 6.

Table S1. 1Kite genomes analyzed. Related to Figure 1A.

Table S2. Accession numbers for proteins used to estimate Zasp phylogeny. Related to Figure 1B.

Table S3. Accession numbers for Zasp66 and Zasp67 orthologs. Related to Figure 2C.