Supplemental Material for Caraballo et al., 2020

Supplementary Figure 1. Branch-specific relaxation in Caenorhabditis UGGTs recognition (top) and catalytic (bottom) domains. Branches are color-coded according to the selection strength parameter k, inferred under the general descriptive RELAX model. Low k values (<1) indicate purifying selection relaxation, whereas high k values (>1) indicate selection intensification.

Supplementary Figure 2. Branch-specific relaxation in vertebrate UGGTs recognition (left) and catalytic (right) domains. Branches are color-coded according to the selection strength parameter k, inferred under the general descriptive RELAX model. Low k values (<1) indicate purifying selection relaxation, whereas high k values (>1) indicate selection intensification.

Supplementary Figure 3 (in Additional File 5). Expression of SpUGGT, Ce-UGGT-a, Ce-UGGT-b and chimeric proteins in alg6/gpt1- cells. Microsomal proteins derived of S.pombe alg6/gpt1 transformed with pREP3X-uggt-b (lane1), pREP3X-uggt-a (lane 2), pREP3X-gpt1+ (lane 3), pREP3X-chimera I (Ce-N-term-uggt-a-C-term-gpt1+c-myc) (lane 4), pREP3X-chimera II (Ce-N-term-uggt-b-C-term gpt1+-c-myc) (lane 5) and pREP3X (lane 6) were analyzed in 8 % SDS PAGE and submitted to Western-blot analysis using a commercial anti c-Myc antibody.

Table S1 (in Additional File 5). Templates and primers used in the PCR amplification of the sequences encoding the N- and C-terminal domains of S. pombe UGGT, Ce-UGGT-a, and Ce-UGGT-b.

Table S2 (in Additional File 5). Templates and primers used in the PCR amplification of the full-length fragments encoding chimeric UGGTs

Table S3 (in Additional File 5). List of DNA primers used in this work

Additional File 1. (xls). Species names, phyla, database and Accession Numbers of the 195 UGGT (and UGGT-like) sequences included in this study. The criteria for excluded sequences is detailed.

Additional File 2. (fas). Unaligned 195 UGGT (and UGGT-like) protein sequences.

Additional File 3. (fas). Alignment of 195 UGGT (and UGGT-like) protein sequences used for phylogenetic inference.

Additional File 4. (doc). Aminoacidic positions corresponding to each UGGT domain taken as reference sequences.

Additional File 5. (doc). Procedures performed in the construction of expression plasmids and list of primer sequences used in this study. Supplementary Figure S3. Western-blot analysis of the expression of the c-Myc labeled full length and chimeric proteins.