Supplemental Material for Campbell et al., 2019

File S1: Mean survival by line for adult males and females exposed to 6 h of anoxia, and mean survival for larvae exposed to 1 h of anoxia.

File S2: Top SNPs (nominal p< 10-5) associated with adult anoxia tolerance.

File S3: Top SNPs (nominal p< 10-5) associated with larval anoxia tolerance.

Figure S1: The correlation between the proportion of larvae that exhibit escape behavior and the proportion of larvae surviving 1 h of anoxia. R=0.16, p=0.047.

Figure S2: Quantile-Quantile plots for adult anoxia tolerance.

Figure S3: Quantile-Quantile plots for larval anoxia tolerance.

Figure S4: Significant SNPs identified in the GWAS are plotted for adults (A) and larvae (B). Effect sizes normalized by the phenotypic standard variation (α/p) are plotted in the top panel. The middle panel shows the minor allele frequencies (MAF) for each significant SNP. P-values plotted as -log10(p) shown in the bottom panel for only SNPs significant at p< 10-5. For adults, colors represent significant SNPs for each of the four adult traits analyzed (Female: red, male: blue, pooled sex average: purple, difference between sexes: black).

Figure S5: Venn diagram showing the overlap of target genes between all adult and larval phenotypes.

Table S1: Correlations between anoxia tolerance and other phenotypes measured in the DGRP. Males and females were regressed individually to phenotypes where sex was listed along with line. Pooled Sex represents the correlations between anoxia tolerance pooled by sex and other DGRP phenotypes in which sex could not be determined (larval and embryo). DGRP phenotypes that included measurements of both sexes or were measured in a stage where sex could not be determined (larvae and embryo) were averaged by line and the correlated to larval anoxia tolerance, represented in the Larvae column. Significance at p<0.001***, p<0.01**, p<0.05*

Table S2: ANOVAs for the effects of Wolbachia infection and inversions on anoxia tolerance

Table S3: Results from the gene ontology enrichment analysis for adult anoxia tolerance.

Table S4: Results from the gene ontology enrichment analysis for larval anoxia tolerance.

Table S5: Results of the likelihood ratio test comparing the effects of RNAi-mediated knockdown on adult anoxia tolerance each gene and sex. Significance at p<0.001***, p<0.01**, p<0.05*

Table S6: Results of the likelihood ratio test comparing the effects of RNAi-mediated knockdown on adult anoxia tolerance between males and females. Significance at p<0.001***, p<0.01**, p<0.05*

Table S7: Results of the likelihood ratio test comparing the effects of RNAi-mediated knockdown on larval anoxia tolerance. p<0.001***, p<0.01**, p<0.05*