Supplemental Material for Bui and Li, 2019

2019-04-26T13:54:10Z (GMT) by Duyen T. Bui Joachim Li

Figures S1-S7 contains the following supplementary figures:

Figure S1 shows re-replication profiles for representative re-replicating strains containing the lys2InsEA14-CAN1 mutagenesis reporter cassette inserted at distinct chromosomal locations

Figure S2 shows basal and re-replication induced gene amplification rates for the strains analyzed in Fig. 2

Figure S3 shows basal and re-replication induced reversion rates for lys2InsEA10 inserted at Site 1

Figure S4 shows basal and re-replication induced gene amplification rates for the strains analyzed in Fig. 3

Figure S5 shows basal and re-replication induced mutations rates for lys2InsEA14 and CAN1 inserted at Site 1 in mismatch repair mutants

Figure S6 shows basal and re-replication induced gene amplification rates for mismatch repair mutant strains analyzed in Fig. 6 and Fig. S5

Figure S7 shows basal and re-replication induced gene amplification rates for REV3 and rev3∆ strains analyzed in Fig. 7

Tables S1-S21 contains the following supplementary tables:

Table S1 contains the list of oligonucleotides used in this study

Table S2 contains the list of plasmids used in this study

Table S3 contains the list of yeast strains used in this study

Table S4 contains the basal and galactose induced rates of Lys+ graphed in Fig. 2B

Table S5 contains the basal and galactose induced rates of Ura+ graphed in Fig. S2

Table S6 contains the basal and galactose induced rates of Lys+ graphed in Fig. S3

Table S7 contains the basal and galactose induced rates of CanR graphed in Fig. 2C

Table S8 contains the basal and galactose induced rates of Lys+ graphed in Fig. 3A (pol2-4)

Table S9 contains the basal and galactose induced rates of CanR graphed in Fig. 3B (pol2-4)

Table S10 contains the basal and galactose induced rates of Ura+ graphed in Fig. S4 (pol2-4)

Table S11 contains the basal and (galactose induced rates of Lys+Ura+)/(galactose induced rates of Ura+) graphed in Fig 4B (pol2-4)

Table S12 contains the sequence of can1R mutations identified in this study

Table S13 contains the basal and galactose induced rates of Lys+ graphed in Fig. 6 & Fig. S5A (msh2∆)

Table S14 contains the basal and galactose induced rates of Lys+ graphed in Fig. S5A (mlh1∆)

Table S15 contains the basal and galactose induced rates of Ura+ graphed in Fig. S6A (msh2∆)

Table S16 contains the basal and galactose induced rates of Ura+ graphed in Fig. S6B (mlh1∆)

Table S17 contains the basal and galactose induced rates of CanR graphed in Fig. S5B (msh2∆)

Table S18 contains the basal and galactose induced rates of CanR graphed in Fig. S5B (mlh1∆)

Table S19 contains the basal and galactose induced rates of Lys+ graphed in Fig. 7A (rev3∆)

Table S20 contains the basal and galactose induced rates of CanRg raphed in Fig. 7B (rev3∆)

Table S21 contains the basal and galactose induced rates of Ura+ graphed in Fig. S7 (rev3∆)