Supplemental Material for Brown et al., 2020

The detection and discrimination of chemical cues can have marked effects on organismal survival and reproduction, eliciting attractive or aversive behavior. To gain insight into mechanisms mediating this hedonic valence, we applied divergent artificial selection for Drosophila melanogaster olfactory behavior. We also tested correlated responses to selection by testing behavioral responses to other odorants and life history traits. Behavioral responses to additional odorants, food consumption and lifespan varied with selection regime and, at times, sex. Differential gene expression analyses revealed functional enrichment of gene ontology terms, including cell-cell adhesion and sulfur compound metabolic process, the latter including glutathione S-transferase genes.