Supplemental Material for Lustyk et al., 2019
Diana Lustyk
Slavomír Kinský
ullrich kristian
Michelle Yancoskie
Lenka Kašíková
Vaclav Gergelits
Radislav Sedlacek
Yingguang Frank Chan
Linda Odenthal-Hesse
Jiří Forejt
Petr Jansa
10.25386/genetics.9874460.v2
https://gsajournals.figshare.com/articles/dataset/Supplemental_Material_for_Lustyk_et_al_2019/9874460
<p>Figure S1 shows male DMC1
hotspot activity in Hstx2 locus.</p>
<p>Figure S2 contains examples of <i>Fmr1nb</i> expression at mRNA and protein level.</p>
<p>Figure S3 shows domain structure of FMR1NB.</p>
<p>Figure S4 shows comparison of litter size generated by B6.DX.1s and B6.DX.1s.<i>Fmr1nb<sup>-</sup></i>
males.</p>
<p>Figure S5 shows comparison of fertility parameters achieved by B6.<i>Fmr1nb</i><sup>-</sup> and B6.DX.1s.<i>Fmr1nb</i><sup>-</sup> males
and their wild type counterparts.</p>
<p>Figure S6 shows frequency of apoptotic cells in testes of B6.<i>Fmr1nb</i><sup>-</sup> and B6.DX.1s.<i>Fmr1nb</i><sup>-</sup> males.</p>
<p> </p>
<p>Table S1
contains microsatellite markers used for genotyping the X chromosome.</p>
<p>Table S2 contain
individual molecules report from DNA samples used for optical maps.</p>
<p>Table S3 shows
parameters of individual reference assemblies</p>
<p> For optical mapping.</p>
<p>Table S4 is composed of xx sub-tables each of them containing
data source for indicated figures and tables.</p>
<p>Table S5 contains Hstx2 candidate genes. </p>
<p>Table S6 shows fertility parameters for hybrid males carrying
either wild type or null mutant of <i>Fmr1nb</i>
gene.</p>
2019-10-10 19:49:10
Speciation – Hybrid sterility X2 – Prdm9 - Bionano optical mapping – SPO11Cas9 transgene - Fmr1nb
Speciation
Hybrid sterility X2
Prdm9
Bionano optical mapping
SPO11Cas9 transgene
Fmr1nb
Genetics