Supplemental Material for Lustyk et al., 2019 Diana Lustyk Slavomír Kinský ullrich kristian Michelle Yancoskie Lenka Kašíková Vaclav Gergelits Radislav Sedlacek Yingguang Frank Chan Linda Odenthal-Hesse Jiří Forejt Petr Jansa 10.25386/genetics.9874460.v2 https://gsajournals.figshare.com/articles/dataset/Supplemental_Material_for_Lustyk_et_al_2019/9874460 <p>Figure S1 shows male DMC1 hotspot activity in Hstx2 locus.</p> <p>Figure S2 contains examples of <i>Fmr1nb</i> expression at mRNA and protein level.</p> <p>Figure S3 shows domain structure of FMR1NB.</p> <p>Figure S4 shows comparison of litter size generated by B6.DX.1s and B6.DX.1s.<i>Fmr1nb<sup>-</sup></i> males.</p> <p>Figure S5 shows comparison of fertility parameters achieved by B6.<i>Fmr1nb</i><sup>-</sup> and B6.DX.1s.<i>Fmr1nb</i><sup>-</sup> males and their wild type counterparts.</p> <p>Figure S6 shows frequency of apoptotic cells in testes of B6.<i>Fmr1nb</i><sup>-</sup> and B6.DX.1s.<i>Fmr1nb</i><sup>-</sup> males.</p> <p> </p> <p>Table S1 contains microsatellite markers used for genotyping the X chromosome.</p> <p>Table S2 contain individual molecules report from DNA samples used for optical maps.</p> <p>Table S3 shows parameters of individual reference assemblies</p> <p> For optical mapping.</p> <p>Table S4 is composed of xx sub-tables each of them containing data source for indicated figures and tables.</p> <p>Table S5 contains Hstx2 candidate genes. </p> <p>Table S6 shows fertility parameters for hybrid males carrying either wild type or null mutant of <i>Fmr1nb</i> gene.</p> 2019-10-10 19:49:10 Speciation – Hybrid sterility X2 – Prdm9 - Bionano optical mapping – SPO11Cas9 transgene - Fmr1nb Speciation Hybrid sterility X2 Prdm9 Bionano optical mapping SPO11Cas9 transgene Fmr1nb Genetics