10.25386/genetics.9767519.v1
Verena A. Kottler
Verena A.
Kottler
Romain Feron
Romain
Feron
Indrajit Nanda
Indrajit
Nanda
Christophe Klopp
Christophe
Klopp
Kang Du
Kang
Du
Susanne Kneitz
Susanne
Kneitz
Frederik Helmprobst
Frederik
Helmprobst
Dunja K. Lamatsch
Dunja K.
Lamatsch
Céline Lopez-Roques
Céline
Lopez-Roques
Jerôme Lluch
Jerôme
Lluch
Laurent Journot
Laurent
Journot
Hugues Parrinello
Hugues
Parrinello
Yann Guiguen
Yann
Guiguen
Manfred Schartl
Manfred
Schartl
Supplemental Material for Kottler et al., 2019
GSA Journals
2019
mosquitofish
sex determination
pool sequencing
gipc1
sox9b
Genetics
2019-11-08 15:04:29
Dataset
https://gsajournals.figshare.com/articles/dataset/Supplemental_Material_for_Kottler_et_al_2019/9767519
Figure S1 presents the results of the RAD-sequencing analysis. Figure S2 shows the analysis of <i>G. affinis </i>and <i>G. holbrooki </i>pooled-sequencing reads aligned to the genome of <i>X. maculatus </i>using 10 kb windows. Figure S3 represents a molecular phylogeny of fish <i>sox9</i>. Figure S4 are alignments of male <i>G. holbrooki </i>and <i>G. affinis </i><i>gipc1</i>and <i>sox9b </i>alleles and protein sequences. Figure S5 are multiple species alignments of Gipc1 and Sox9 protein sequences. Figure S6 contains PCR results demonstrating that <i>sox9bY </i>was generated by allelic diversification. Figure S7 contains pictures of melanic and non-melanic <i>G. holbrooki</i>. Table S1 gives information on the fish used for pool sequencing. Table S2 presents the results of the allele-specific PCRs. Table S3 contains the annotated loci within the 100 kb windows with the highest density of sex-specific SNPs. Table S4 contains the sex chromosome genotypes of F<sub>1 </sub>fish (pedigree WLC20074) from a cross between a <i>G. affinis </i>female (WLC1313) and a <i>G. holbrooki </i>male (WLC2514) determined by PCR. Table S5 gives information on the number of offspring of non-melanic females and non-melanic males derived from melanic strains.