Supplemental Material for Campbell et al., 2019
Jacob Campbell
Paula Overby
Alyx Gray
Hunter Smith
Jon F. Harrison
10.25387/g3.8316494.v1
https://gsajournals.figshare.com/articles/dataset/Supplemental_Material_for_Campbell_et_al_2019/8316494
<p><b>File S1: </b>Mean
survival by line for adult males and females exposed to 6 h of anoxia, and mean
survival for larvae exposed to 1 h of anoxia.</p>
<p> </p>
<p><b>File S2: </b>Top SNPs
(nominal <i>p</i>< 10<sup>-5</sup>) associated with adult anoxia tolerance.</p>
<p> </p>
<p><b>File S3: </b>Top SNPs
(nominal <i>p</i>< 10<sup>-5</sup>) associated with larval anoxia tolerance.</p>
<p> </p>
<p><b>Figure S1: </b>The
correlation between the proportion of larvae that exhibit escape behavior and
the proportion of larvae surviving 1 h of anoxia. <i>R</i>=0.16, <i>p</i>=0.047.</p>
<p><b>Figure
S2:</b> Quantile-Quantile
plots for adult anoxia tolerance.</p>
<p><b>Figure S3:</b> Quantile-Quantile
plots for larval anoxia tolerance.<b></b></p>
<p><b>Figure S4: </b>Significant
SNPs identified in the GWAS are plotted for adults (A) and larvae (B). Effect
sizes normalized by the phenotypic standard variation (α/p) are plotted in the top
panel. The middle panel shows the minor allele frequencies (MAF) for each
significant SNP. P-values plotted as -log10(<i>p</i>)
shown in the bottom panel for only SNPs significant at <i>p</i>< 10<sup>-5</sup>. For adults, colors represent significant
SNPs for each of the four adult traits analyzed (Female: red, male: blue,
pooled sex average: purple, difference between sexes: black). </p>
<p><b>Figure S5: </b>Venn
diagram showing the overlap of target genes between all adult and larval
phenotypes. </p>
<p> </p>
<p> </p>
<p><b>Table S1:</b> Correlations between anoxia tolerance and
other phenotypes measured in the DGRP. Males and females were regressed
individually to phenotypes where sex was listed along with line. Pooled Sex
represents the correlations between anoxia tolerance pooled by sex and other
DGRP phenotypes in which sex could not be determined (larval and embryo). DGRP
phenotypes that included measurements of both sexes or were measured in a stage
where sex could not be determined (larvae and embryo) were averaged by line and
the correlated to larval anoxia tolerance, represented in the Larvae column.
Significance at p<0.001***, p<0.01**, p<0.05*</p>
<p> </p>
<p><b>Table S2:</b> ANOVAs
for the effects of Wolbachia infection and inversions on anoxia tolerance</p>
<p><b>Table S3: </b>Results
from the gene ontology enrichment analysis for adult anoxia tolerance.</p>
<p><b>Table S4: </b>Results
from the gene ontology enrichment analysis for larval anoxia tolerance.</p>
<p><b>Table S5:</b> Results
of the likelihood ratio test comparing the effects of RNAi-mediated knockdown
on adult anoxia tolerance each gene and sex. Significance at p<0.001***,
p<0.01**, p<0.05*</p>
<p><b>Table
S6: </b>Results
of the likelihood ratio test comparing the effects of RNAi-mediated knockdown
on adult anoxia tolerance between males and females. Significance at
p<0.001***, p<0.01**, p<0.05*</p>
<p><b>Table
S7: </b>Results
of the likelihood ratio test comparing the effects of RNAi-mediated knockdown
on larval anoxia tolerance. p<0.001***, p<0.01**, p<0.05*</p>
2019-07-16 16:02:11
Drosophila melanogaster animal model
stress tolerance
hypoxia
DGRP GWA study
DGRP
Animal Physiological Ecology
Comparative Physiology
Genetics
Physiology
Population, Ecological and Evolutionary Genetics
Quantitative Genetics (incl. Disease and Trait Mapping Genetics)