10.25386/genetics.8031848.v1
Karen Lange
Karen
Lange
Aly Suleman
Aly
Suleman
Martin Srayko
Martin
Srayko
Supplemental Material for Lange, Suleman, and Srayko, M., 2019
GSA Journals
2019
PP2A phosphatase
Ska complex
C. elegans
centrosome
kinetochore
microtubule
Aurora kinase
TPX2
Spindle assembly
Cell Biology
2019-04-24 14:08:21
Dataset
https://gsajournals.figshare.com/articles/dataset/Supplemental_Material_for_Lange_Suleman_and_Srayko_M_2019/8031848
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<p>Figure S1 shows anti-Ska immunofluorescence in one-cell embryos treated with control or ska-1 RNAi. Figure S2 shows KLP-7-GFP in the one-cell embryo is not altered by ska-1(RNAi) or ska-3(RNAi). Figure S3 shows Ska mutations do not restore microtubule levels in rsa-1(or598) embryos. Figure S4 shows TPXL-1-GFP at the centrosome is not altered by ska-1(RNAi). Figure S5 shows Ska is required to form a tight metaphase plate. Figure S6 shows the rsa-1(or598) spindle-collapse phenotype is partially rescued by loss of Ska function. Figure S7 shows the rsa-1(or598) spindle-collapse phenotype is partially rescued by loss of KMN kinetochore components and loss of EBP-2. Table S1 contains information on C. elegans strains used in this study. Table S2 contains information on cloning primers used in this study.</p>