Marsac, Roxane Pinson, Benoît Saint-Marc, Christelle Olmedo, María Artal-Sanz, Marta Daignan-Fornier, Bertrand Gomes, José-Eduardo Supplemental Material for Marsac et al., 2019 Purine biosynthesis pathways are highly conserved in <i>C. elegans</i>. We describe the composition of the purine pathways in <i>C. elegans</i>, based on sequence homology with both yeast and humans and present HPLC metabolic profiling standard in worms, particularly concerning nucleotide metabolism. We present developmental phenotypes in <i>C. elegans</i> purine mutants, particularly Adenylosuccinate lyase deficiency: vulval defects, fertility, embryonic lethality and post-embryonic developmental timing. We further present the metabolic profiles of <i>C. elegans</i> treated with AICAR - both wild-type and mutants deficient for the purine <i>de novo</i> synthesis pathway. Finally we present data describing the analysis of neuromuscular function in purine mutants: Aldicard and Levamisole treatments and mechanosensory response test. biosynthetic pathway;nucleotide metabolisms;animal metabolism;muscle structure;post-embryonic development;nematode models;germline proliferation;ADSL deficiency;PPAT;ATIC;adenylosuccinate lyase;Genetics;Cell Metabolism;Developmental Biology 2019-01-30
    https://gsajournals.figshare.com/articles/figure/Supplemental_Material_for_Marsac_et_al_2019/7642871
10.25386/genetics.7642871.v1