Supplemental Material for Després et al., 2018
Philippe C Després
Alexandre K Dubé
Lou Nielly-Thibault
Nozomu Yachie
Christian R Landry
10.25387/g3.6936683.v1
https://gsajournals.figshare.com/articles/dataset/Supplemental_Material_for_Despr_s_et_al_2018/6936683
Figure S1 shows sequencing of ADE1 loci after Target-AID base editing using double selection. Figure S2 shows Sanger chromatograph titration curve based on different allelic frequencies in the population prior to sequencing. Figure S3 showcases an example of how the lethality of a mutation can influence the observed mutation rate in the population. Figure S4 shows another example of Cas9 mediated chromosomal rearrangement following multiplex editing. Table S1 shows the different plasmids used in this study. Table S2 shows the oligonucleotides used in this study. Table S3 shows a summary of the different gRNA sequences used in this study. Table S4 shows the recipes for synthetic media used in this study.<br>
2018-08-09 18:32:18
Yeast Genome editing
CRISPR-Cas9
Target-AID Base editing
Loss of function screens
Chromosomal fusions
Synthetic Biology
Genetics
Biological Techniques
Biological Engineering