Supplemental Material for Zhang et al., 2018
Zijing Zhang
Amber R. Krauchunas
Stephanie Huang
Mariana F. Wolfner
10.25387/g3.6820784.v2
https://gsajournals.figshare.com/articles/dataset/Supplemental_Material_for_Zhang_et_al_2018/6820784
<p><a><b>Figure
S1 RNAi knockdown of Class 3 and 4 genes led to defects in oocyte development</b></a>. Ovaries dissected from 3-5 days old
control or knockdown females were stained for DNA (red) using propidium iodide.
A summary of the ovarian phenotypes can be found in Table S3. Images are
arranged according to observed stage of oogenesis defect, from early stage to
late stage. Scale bar: 50 µm.</p>
<p> </p>
<p><a><b>Figure
S2. Maternal knockdown of 12 genes led to reduction and elimination of egg
hatchability that recapitulated previously reported maternal effect phenotypes.</b></a> 2-4 hr old embryos from knockdown
females, stained for tubulin (green) and DNA (red). For each set of images, the
image on the right shows a close-up view of the spindle (marked by the arrow on
the left). Images are organized in alphabetical order. The AttP2 panel, the
control for comparison, is the same as in Fig. 2. Scale bar: oocyte image 50
µm, enlarged image 10 µm.</p>
<p> </p>
<p><a><b>Figure
S3.</b></a> <b>Fertilization was not significantly
impacted in the early arrest knockdown embryos of the 27 genes</b> Sperm tail (green;
arrow) was detected in the stage 1-2 arrested embryos produced by RNAi females for
27 genes. The images are ordered in alphabetical order, by gene name. Scale
bar: 50 µm.</p>
<p> </p>
<p><a><b>Figure
S4. Translation of <i>smg</i> in unfertilized eggs produced by control and RNAi
females of 27 early maternal effect candidates</b></a>. Among the 27 genes examined, Smg protein
was detected in the activated eggs produced by RNAi females of 26 genes.
Translation of Smg protein was not detected in the activated eggs produced by <i>plu</i> knockdown females.<br><br><b>Table S1</b> <b>- S7</b><br></p>
2018-07-30 19:50:06
egg activation
oogenesis
embryogenesis
maternal protein
phosphorylation state
Drosophila melanogaster
Genetics