10.25386/genetics.6205691.v1
Herman K. Edskes
Herman K.
Edskes
Maryam Mukhamedova
Maryam
Mukhamedova
Bouke K. Edskes
Bouke K.
Edskes
Reed B. Wickner
Reed B.
Wickner
Supplemental Material for Edskes et al., 2018
GSA Journals
2018
LUG1/YLR352W
F-box protein
Ure2p
nitrogen catabolite repression
Hap4p
prion
URE3
gln1
Genetics
2018-05-16 16:24:15
Dataset
https://gsajournals.figshare.com/articles/dataset/Supplemental_Material_for_Edskes_et_al_2018/6205691
Supplemental Methods_figures_tables.pdf - Provides a detailed description of the culture conditions,
induction of <i>Hermes</i> transposition,
selection of colonies carrying a transposition, extraction of cellular DNA, PCR
amplification and isolation of the junction points between transposon and
chromosomal insertion site, next-generation sequencing of these sites, and
analysis of the data by visual display and by counting insertions per open
reading frame. <br><br> Exon Intron Counts.xlsx - Gives the insertions in
every yeast open reading frame, distinguishing exons from introns where
appropriate.<br><br>Sorted Hits.xlsx - Gives prominent hits sorted by functional
group, “TY gag-pol Counts.xlsx comparing insertions in the Ty retrotransposons
at different locations in the genome. <br><br>Count Insertions in ORFs and
Introns.txt - The Python program used for counting insertions. <br><br>LUGsIGV-InsertDistributions.pptx - Is a slide show of insert distributions in
each of 500 genes for which insertions were recovered more frequently in
[ure-o] than in [URE3] cultures.