Mishra, Rakesh Kunar, Rohit Manda, Lolitika Pankaj Alone, Debasmita Chandrasekharan, Shanti Tiwari, Anand Krishna Tapadia, Madhu Gwaldas Mukherjee, Ashim Roy, Jagat Kumar Supplemental Material for Mishra et al., 2020 <p></p><p><b>Supplementary Figure S1 </b>shows the reversion analysis by the excision of <i>P</i>-elements from mutant alleles of <i>DCP2</i>.</p> <p><b>Supplementary Figure S2 </b>shows the morphological defects exhibited by escapees of adult fly trans-heterozygous for <i>P{GT1}DCP2<sup>BG01766</sup>/l(3)tb</i>. </p> <p><b>Supplementary Figure S3</b> shows the defects in compound eyes of the escapees having heterozygous genetic background of the mutant <i>l(3)tb </i>with lethal <i>P-</i>insertion allele <i>DCP2<sup>BG01766</sup></i>.</p> <p><b>Supplementary Figure S4</b> shows the tumorous phenotype observed in larval brain and wing imaginal discs in trans-heterozygotes <i>l(3)tb /PBac{RB}DCP2<sup>e00034</sup></i> and <i>l(3)tb<sup> </sup>/P{GT1}DCP2<sup>BG01766</sup></i> similar to homozygous <i>l(3)tb</i> mutants.</p> <p><b>Supplementary Figure S5</b> shows the results of screening of <i>DCP2</i> in the <i>l(3)tb </i>mutants, using overlapping primers. </p> <p><b>Supplementary Figure S6 </b>shows the<b> </b>schematic representation of the convergent bidirectional primer walking adopted for sequencing and alignment of the large amplicon obtained at the candidate region in <i>DCP2<sup>l(3)tb</sup></i> homozygotes and the reads obtained on sequencing with each of the four primers.</p> <p><b>Supplementary Figure S7 </b>shows<b> </b>that the effects of ubiquitous knockdown of <i>Diablo </i>does not lead to any developmental anomaly and does not affect survival of the driven progeny. </p> <p><b>Supplementary Table S1 </b>depicts the complementation status of <i>l(3)tb</i> with cytologically mapped deletion lines. </p> <p><b>Supplementary Table S2 </b>shows the complementation analysis of <i>l(3)tb </i>with lethal transposon insertion lines.<b></b></p> <p><b>Supplementary Table S3 </b>lists the primers used for characterizing deletion in <i>Df(3L)RM95</i>.</p> <p><b>Supplementary Table S4 </b>shows the overlapping set of primers for <i>DCP2</i> and thermal cycler conditions of annealing temperature and extension time for each primer pair to amplify the genomic region of <i>DCP2</i> gene in the homozygous <i>l(3)tb</i> mutant.</p> <p><b>Supplementary Table S5 </b>lists the overlapping set of primers to amplify the genomic region in <i>DCP2</i> gene for the region covered by the DCP2_P19 set of primers in the homozygous <i>l(3)tb</i> mutant.</p> <p><b>Supplementary Table S6 </b>lists the overlapping set of primers and thermal cycling conditions used to amplify the complete 5’UTR of genomic region in <i>DCP2</i> gene in the homozygous <i>l(3)tb</i> mutant. </p><br><p></p> DCP2;tumour suppressor;Drosophila;genetic mapping;forward genetics;Cancer;Cell Biology;Cell Development, Proliferation and Death;Genetics 2020-06-26
    https://gsajournals.figshare.com/articles/dataset/Supplemental_Material_for_Mishra_et_al_2020/12568265
10.25387/g3.12568265.v1