Sae-Lee, Wisath Scott, Luisa L. brose, lotti Encarnacion, Aliyah J. shi, ted Kore, Pragati oyibo, Lashaun Ye, Congxi Rozmiarek, Susan K. Pierce, Jonathan T. Supplemental Material for Sae-Lee et al., 2020 <p></p><p><b>Figure S1.</b> Representative images of degenerating HSN neurons. <b>A-C,</b> Degenerated HSN neurons had dim (arrow heads) or barely visible (dotted circle) cell bodies. <b>D-G,</b> Earlier evidence of HSN neuron degeneration was visible as blebbing of the cell body (arrows). <b>H-I</b>, Processes of degenerating HSN neurons also showed beading (H, arrows) that was not visible in healthy HSN neurons (I). Autofluorescence from intestine bright in panels H and I due to higher gain to visualize HSN axon. VC4 and VC5 neurons fluorescing out of focus in panels B-F. </p> <p> </p> <p><b>Figure S2.</b> APP::mCherry is expressed throughout the nervous system.<b> A</b> DIC and fluorescent photomicrographs at 40X along the length of the worm. A C-terminal mCherry tag shows APP expression. As expected, we found that APP expressed under a pan-neuronal promoter was seen throughout the entire nervous system including the nerve ring and along the ventral nerve cord in the absence (left) and presence (right) of exAPOE4. <b>B </b>Enlarged photomicrographs of the ventral nerve cord taken mid-body show that APP is expressed in a punctate pattern in the VCs both when APOE4 expression is absent (top) and present (bottom). <b>C</b> Enlarged photomicrographs show that APP is expressed in a punctate pattern in the HSNs.</p> <p> </p> <p><b>Figure S3.</b> <i>APOE3 </i>and<i> APOE4</i> transgene levels. <b><i>A,</i></b> Semi-quantitative analysis of level of <i>APOE</i> transgene arrays in genomic DNA. Average band intensities of three independent genomic preparations from five worms/strain following PCR for human APOE presented as mean ± SEM. No significant differences were found when comparing levels for APOE3 vs APOE4 strains after correcting for multiple comparisons. <b><i>B,</i></b> Expression of <i>APOE </i>determined by RT-qPCR of mRNA isolated from 3 (integrated) or 9 (extrachromosomal) near-starved 6-cm plates of worms. DCt values reveal little difference in gene expression between strains. Values represent the mean ± SE of two independent biological replicates with three technical replicates each. Data is represented as gene expression relative to the reference control gene <i>tba-1</i>. </p> <p> </p> <p><b>Table S1</b>. List of strains used throughout our study arranged by Figure.</p><br><p></p> APP;apoe4;C. elegans;neurodegeneration;Neurogenetics 2020-06-24
    https://gsajournals.figshare.com/articles/dataset/Supplemental_Material_for_Sae-Lee_et_al_2020/12493769
10.25387/g3.12493769.v1