Supplemental Material for McHugh et al., 2020
Daniel R. McHugh
Elena Koumis
Paul Jacob
Jennifer Goldfarb
Michelle Schlaubitz-Garcia
Safae Bennani
Paul Regan
Prem Patel
Matthew J. Youngman
10.25387/g3.11964351.v2
https://gsajournals.figshare.com/articles/figure/Supplemental_Material_for_McHugh_et_al_2020_post_accept_/11964351
These data support our functional characterization of DAF-16 during aging in <i>Caenorhabditis elegans</i>. We monitored the survival of <i>daf-16</i> mutant larvae and adults upon infection with the bacterial pathogen <i>P. aeruginosa</i> to investigate a possible role for DAF-16 in host defense. We also examined age-dependent changes in gene expression, including transcriptional targets of DAF-16, by RNAseq, by qRT-PCR, and by <i>in vivo</i> reporter analysis.
2020-03-11 14:15:26
C. elegans
innate immunity
aging
DAF-16
SMK-1
Innate Immunity
Genetics
Invertebrate Biology