Supplemental Material for McHugh et al., 2020 Daniel R. McHugh Elena Koumis Paul Jacob Jennifer Goldfarb Michelle Schlaubitz-Garcia Safae Bennani Paul Regan Prem Patel Matthew J. Youngman 10.25387/g3.11964351.v2 https://gsajournals.figshare.com/articles/figure/Supplemental_Material_for_McHugh_et_al_2020_post_accept_/11964351 These data support our functional characterization of DAF-16 during aging in <i>Caenorhabditis elegans</i>. We monitored the survival of <i>daf-16</i> mutant larvae and adults upon infection with the bacterial pathogen <i>P. aeruginosa</i> to investigate a possible role for DAF-16 in host defense. We also examined age-dependent changes in gene expression, including transcriptional targets of DAF-16, by RNAseq, by qRT-PCR, and by <i>in vivo</i> reporter analysis. 2020-03-11 14:15:26 C. elegans innate immunity aging DAF-16 SMK-1 Innate Immunity Genetics Invertebrate Biology