Supplemental Material for Randhawa et al., 2020
Imtiaz A.S. Randhawa
Brian M. Burns
Michael R. McGowan
Laercio R. Porto-Neto
Ben J. Hayes
Ryan Ferretti
Karen M. Schutt
Russell E. Lyons
10.25387/g3.10423904.v1
https://gsajournals.figshare.com/articles/dataset/Supplemental_Material_for_Randhawa_et_al_2020/10423904
<p><b>Table S1</b>
Comparison of SNP-based current poll testing (CPT) and the optimized poll
testing (OPT) assays on 20,636 samples for the frequency of “No results” in
various cattle breeds.</p>
<p><b><br></b></p><p><b>Table S2</b>
List of 14 microsatellites mapped within the Poll locus on chromosome 1.</p>
<p><b><br></b></p><p><b>Table S3</b>
Call rate of 10 SNPs in different cattle breeds and cross-bred populations.</p>
<p><b><br></b></p><p><b>Table S4</b>
Results of targeted DNA sequences of 55 Brahman samples showing 60bp genome
region around SNP rs800947704[C/T=Y] (marked as 0) and upstream variant SNP
rs381418143 [A/G=R] (marked as -3). Two types of errors were observed in the
genotyping assays, 1: genotype failed (--) when -3=G (yellow highlights), 2:
wrong genotypes when -3=R and 0=Y (purple highlights).</p>
<p><b><br></b></p><p><b>Figure S1</b>
Distribution (%) of MSAT and SNP based poll gene testing results for Horned
(HH), Hybrid (HP), Polled (PP) and failing (“Not Determined” or “No Results” )
samples across 10 Australian beef breeds (n=16,941).</p>
<p><b><br></b></p><p><b>Figure S2</b>
Breed-wise distribution (%) of Optimized Poll Testing (OPT) assay results (HH =
Horned, HP = Hybrids, PP = Polled, No Results = Failed assay) in first column
and Allele Frequencies (AF) of Horn (H), Poll-Celtic (P<sub>C</sub>) and Poll-Friesian
(P<sub>F</sub>) mutations in column 2 across ten Australian beef breeds (n
= 16,903). Note that presence of H alleles in Angus and P<sub>C</sub>/P<sub>F</sub>
alleles in Wagyu is indicative of cross-bred individuals. Overall, the results
show that polled animals of Australian beef breeds predominantly carry P<sub>C</sub> as
compared to P<sub>F</sub>, except for Shorthorn.</p>
<p><b><br></b></p><p><b>Data
1</b>
Validation population phenotypes and Poll markers genotypes data file: Data_1_project_validation_populations_phenotypes_and_poll_locus_SNPs.csv</p>
<p><b><br></b></p><p><b>Data
2 </b>Commercial
population Poll markers genotypes and BREEDPLAN phenotypes data file: </p>
Data_2_project_poll_locus_10_SNPs_genotypes_and_BREEDPLAN_phenotypes.csv
2019-11-25 16:52:53
poll gene
bovine
dehorning
genetic testing
animal welfare
Animal Breeding
Animal Management
Veterinary Diagnosis and Diagnostics