10.25387/g3.10406519.v1 Elizabeth A. Morton Elizabeth A. Morton Ashley Hall Ashley Hall Elizabeth X. Kwan Elizabeth X. Kwan Calvin Mok Calvin Mok Konstantin Queitsch Konstantin Queitsch Vivek Nandakumar Vivek Nandakumar John Stamatoyannopoulos John Stamatoyannopoulos Bonita J. Brewer Bonita J. Brewer Robert Waterston Robert Waterston Christine Queitsch Christine Queitsch Supplemental Material for Morton et al., 2020 GSA Journals 2019 Ribosomal DNA Repetitive DNA Whole Genome Sequencing Copy Number Variation Genomics 2019-11-22 16:40:56 Dataset https://gsajournals.figshare.com/articles/dataset/Supplemental_Material_for_Morton_et_al_2020/10406519 <p> </p><p>Supplemental methods, figures, and tables accompanying Morton <i>et al</i>. manuscript "Challenges and Approaches to Genotyping Repetitive DNA"</p> <p>File S1 contains supplemental materials and methods and a list of tables</p> <p>Figure S1 contains Southern blots and ethidium bromide staining of CHEF gels</p> <p>Figure S2 contains droplet digital PCR condition optimization</p> <p>Figure S3 contains details of smMIP-based copy number estimation</p> <p>Figure S4 contains details of rDNA locus FISH characteristics for <i>C. elegans</i> strains</p> <p>Table S1 contains metrics from Nextera whole genome sequencing of <i>C. elegans</i> strains</p> <p>Table S2 contains percent error of rDNA copy number estimation for <i>C. elegans</i> strains</p> <p>Table S3 contains results of downsampling <i>C. elegans</i> WGS data</p> <p>Table S4 contains different aligner and trimming comparisons of <i>C. elegans</i> strains rDNA copy number estimate</p> <p>Table S5 contains coordinate of <i>C. elegans</i> single copy regions</p> <p>Table S6 contains WGS counts of <i>C. elegans</i> single copy regions</p> <p>Table S7 contains published yeast strain SRA numbers</p> <p>Table S8 contains yeast diploid strain rDNA estimates</p> <p>Table S9 contains yeast strain SNV genotyping</p> <p>Table S10 contains droplet digital PCR raw data</p> <p>Table S11 contains smMIP data</p> <p>Table S12 contains rDNA FISH summary cell counts</p> <p>Table S13 contains rDNA FISH data</p> <p>Table S14 contains sequences of oligos</p> <p>Table S15 contains band measurements from <i>C. elegans</i> CHEF gels</p>